QUANTITY: 25 mg 

Information: Human CD80 (B7-1) is a costimulatory ligand for CD28 and CTLA-4(1). CD80 is expressed on activated B cells (2).

Molecular Structure: A soluble dimeric fusion protein consisting of the extracellular (173aa) domain of  human CD80 fused to murine IgG2a Fc + hinge (232 aa). Predicted monomeric molecular weight : 51.3 kd. 

Transfectant Cell Line: CHO

Immunochemistry Applications: Blocking Antibody or recombinant protein binding to CD80. Component for ELISA use.

References:1. C.B. Thompson, (1995) Cell 81: 979-982. 2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 682-684.

STORAGE CONDITIONS: Store at 2 - 5^oC. Open under aseptic conditions. Freeze/Thawing is not recommended.

PRODUCT STABILITY: Product should retain activity for at least 12 months after shipping date when stored as recommended. Ship Date:_____________

BUFFER: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. Product was sterile filtered and vialed under aseptic conditions.

PRODUCTION: Human CD80-muIg fusion protein was purified from (low FBS containing) tissue culture supernatant of CHO transfectants.

PERFORMANCE: CD80-muIg was reactive in an Enzyme Immuno-assay utilizing a Goat anti-Mouse Ig coated plate for capture, and CD152-muIg/Biotin recombinant protein (Catalog #501-030) or anti-CD80/Biotinylated antibody (Catalog #110-030) for detection, followed by Streptavidin/HRP and TMB/H₂O₂ substrate chromagen.